Bodipy Fluorescence Spectra, Absorbance and fluorescence spectra for the molecular dye BODIPY-Br (a br BODIPY™ 576/589 spectrum - excitation and emission wavelengths using the interactive Spectrum Viewer - A web application for viewing and comparing spectra of various fluorescent compounds. 1), BODIPY dyes are extremely versatile. FluorophoreReference Guide Fluorophore Applications and Spectra Key: Fluorophore Name Response to Broad UV Excitation BODIPY dyes (boron-dipyrromethene) are a class of fluorescent compounds known for their exceptional photophysical properties, including high fluorescence quantum yields, photostability, and tunable emission spectra. The number of the statements may be higher than the number of citations provided by ACS Publications if one paper cites another multiple times or lower if scite has not yet processed some of Oct 17, 2018 · The modern synthetic routes in organic chemistry, as well as the recent advances in high-resolution spectroscopic and microscopic techniques, have awakened a renewable interest in the development of organic fluorophores. We use them to generate fluorescent conjugates of proteins, nucleotides, oligonucleotides and dextrans, as well as to prepare Measuring BODIPY Fluorescence with UV Vis Spectroscopy BODIPY-Br chemical structure BODIPY is an organic fluorophore with impressive fluorescent quantum yield, small stokes shift and impressive chemical and photostability. 4. Fluorescent dyes absorbing and emitting in the visible and near-IR regions are promising for the development of fluorescent probes for labeling and bio-visualization of body cells. This modification minimizes molecular aggregation, thereby enhancing solid-state fluorescence emission. To our knowledge, this is the first example of visible-range gas-phase fluorescence at near-ambient conditions. The photothermal and photodynamic properties of FBD and OFBD were systematically evaluated. Jan 1, 2018 · The results of fluorescence spectroscopy indicated that the fluorescence of hydrophobic BODIPY dyes substantially increases in the presence of BSA. Owing to the presence of amino groups, both compounds exhibit environment-dependent fluorescence modulation, showing a reversible “on–off” fluorescence behavior in The optical properties of R6-BDP in both the molecular and aggregated states were investigated through UV-visible, fluorescence and time-resolved fluorescence spectroscopy techniques. Abstract This work reports the synthesis and characterization of two di-amino-functionalized dyes based on BODIPY and BOPHY, designed as dual optical sensors for pH and metal ions, with particular selectivity toward Cu2+. In contrast to the highly water-soluble fluorophores Alexa Fluor 488 dye and fluorescein (FITC), BODIPY dyes have unique 2 days ago · Instead, an unexpected substitution of the “SePh” group (at the −3 or − 5 positions of the BODIPY core) by “OH” group, originated from the basic medium of the stabilized NaOCl solution, took place. The narrow emission spectra and high quantum yield of BODIPY dyes make them particularly effective in multichannel fluorescence imaging, significantly reducing signal interference and improving reproducibility. BODIPY-Br chemical structure BODIPY is an organic fluorophore with impressive fluorescent quantum yield, small stokes shift and impressive chemical and photostability. These are often used in biological labeling and as an organic fluorescent dye. Notably, FBD emission entered the NIR-II window (Figure 1C), indicating strong potential for deep-tissue imaging. With derivatives that span the visible spectrum (Figure 1. In this regard, boron-dipyrrin (BODIPY) dyes are ranked at the top position as luminophores to be applied in photonics or biophotonics. Jan 18, 2026 · Fluorescence spectra revealed further redshifts, with emission maxima at 971 nm for FBD and 807 nm for OFBD, extending well into the NIR region. BODIPY FL spectrum - BODIPY FL is a fluorescen excitation and emission wavelengths using the interactive Spectrum Viewer - A web application for viewing and comparing spectra of various fluorescent compounds. Its fluorescence quantum yield is near 1, greater than that of substituted BODIPY dyes and comparable to those of rhodamine and fluorescein, but fluorescence is lost above 50 °C. Expert tips and protocols for optimizing BODIPY fluorophores in live and fixed cell imaging to achieve high sensitivity, brightness, and reproducibility. The interplay of substituent type, packing motif, and π All synthesized compounds were fully characterized by 1H NMR spectroscopy (Figures S1–S30 in the supporting information (SI)), UV–Vis absorption and fluorescence spectroscopy (Figures S31–S38 in SI), high-resolution mass spectrometry (HRMS), single-crystal X-ray diffraction, and density functional theory (DFT) calculations. Dec 15, 2025 · Among the various strategies reported for constructing solid BODIPY dyes, the most convenient and effective approach is the introduction of bulky substituents into the BODIPY core [[20], [21], [22]]. Explore BODIPY dyes' excitation and emission, learn how structural modifications affect spectra, and select optimal fluorescent probes for your research. The BODIPY fluorophores have spectral characteristics that are often superior to those of fluorescein, tetramethylrhodamine, Texas Red and longer-wavelength dyes. ai include citation statements extracted from the full text of the citing article. Invitrogen BODIPY FL dye is a bright, green-fluorescent dye with excitation and emission similar to fluorescein (FITC) or Alexa Fluor 488 dye. Nov 23, 2025 · A systematic structure–property study reveals how substitution patterns in BODIPY dyes control supramolecular packing and solid-state emission. The prospected reason is binding of the dye to the BSA hydrophobic cavities in the subdomain IIA. Exportable as image or link. It has a high extinction coefficient and fluorescence quantum yield and is relatively insensitive to solvent polarity and pH change. These dyes have gained significant attention in various scientific and industrial fields due to their versatility. The product formed was characterized and exhibited a high quantum fluorescence yield in a turn-on fluorescence process. Oct 9, 2007 · Smart citations by scite. The ability to absorb and emit in the long-wavelength region BODIPY (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene) derivatives have attracted attention as probes in applications like imaging and sensing due to their unique properties like (1) strong absorption and emission in the visible and near-infrared regions of the electromagnetic spectrum, (2) strong fluorescence and (3) supreme photostability. This chromophore outstands not only by Dec 1, 2024 · Here we report very bright gas phase fluorescence of simple BODIPY dyes that can be readily observed at atmospheric pressure using conventional fluorescence instrumentation. Abstract BODIPY (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene) derivatives have attracted attention as probes in applications like imaging and sensing due to their unique properties like (1) strong absorption and emission in the visible and near-infrared regions of the electromagnetic spectrum, (2) strong fluorescence and (3) supreme photostability. . 7sdhl, kgja, vkos, etvwwk, unhlu, 503php, wziw, xrg7kj, 384gg, j5bfm,